SS-31laboratory reference kit

SS-31 (D-Arg-Dmt-Lys-Phe-NH2) is a synthetic tetrapeptide engineered to accumulate selectively at the inner mitochondrial membrane, where it binds cardiolipin and stabilises supercomplex assembly. Published preclinical work has explored SS-31 in cardiac ischaemia–reperfusion models, age-related mitochondrial dysfunction panels, and renal and skeletal-muscle bioenergetic studies.

Because the peptide carries a Dmt (dimethyl-tyrosine) residue and a D-arginine, SS-31 is significantly more synthetically demanding than standard L-amino-acid peptides — which is why batch testing and supplier selection matter even more than usual.

Each SS-31 production lot is independently tested before release.

Janoshik Analytical is the default verification partner for SS-31, with secondary verification on selected batches through additional European laboratories. Per-batch reports are published on the lab reports page and tied to the batch number printed on every vial cap.

Lyophilised SS-31 is supplied as a sterile white powder under inert atmosphere. Hold unopened vials at 2–8 °C; transfer to −20 °C if reconstitution will not happen within 8–12 months. Reconstitute with bacteriostatic water for a two-month (60-day) in-use stability window under refrigeration; protect from prolonged light exposure to preserve the Dmt residue.

Most laboratories work at 1–5 mg/ml stock concentrations. SS-31 is sensitive to repeat freeze–thaw cycles — aliquot the full reconstituted volume before any freeze to preserve potency across multi-week protocols.

SS-31 and MOTS-c are often studied side-by-side in mitochondrial-bioenergetics panels, but they operate via completely distinct mechanisms. SS-31 binds cardiolipin at the inner mitochondrial membrane, stabilising electron-transport-chain supercomplex assembly. MOTS-c is encoded inside mitochondrial DNA and acts through AMPK-linked metabolic signalling.

Stocking both compounds from the same supplier keeps documentation and packaging consistent across a comparative bioenergetics panel.

SS-31 contains a Dmt residue and a D-arginine, both of which are easy to substitute with cheaper L-tyrosine and L-arginine analogues by a careless or dishonest supplier. Mass-spec identity confirmation on every batch is non-negotiable — HPLC alone cannot detect that kind of substitution.

An honest SS-31 supplier publishes the per-batch COA, names the testing laboratory, and prints the batch number on the vial. Regena's consultations team will walk a new buyer through the COA and the reconstitution plan before any order ships.