Hormone · Tripeptide
Testoluten
Also known as Lys-Glu-Asp
A tripeptide (Lys-Glu-Asp) investigated in Leydig cell models for its influence on steroidogenic gene expression and testosterone synthesis.
- Length
- 3 amino acids
- Half-life
- Not well characterised
- Mechanism
- This peptide is studied for its potential to influence steroidogenic pathways and cellular function within in-vitro models of testicular Leydig cells.
- Studied in
- Laboratory studies use it in Leydig cell cultures to investigate its effects on testosterone biosynthesis and the expression of related genes.
About Testoluten
Testoluten is the synthetic tripeptide L-Lysyl-L-Glutamyl-L-Aspartic acid. Its sequence is derived from peptides found in testicular tissue. The synthetic form provides a pure and reproducible material for exploring its biological role in a controlled laboratory environment.
The focus of in-vitro research is its effect on testicular endocrine function, specifically on Leydig cells, which are responsible for producing androgenic hormones. Studies employ immortalized Leydig cell lines (e.g., MA-10, TM3) to analyze changes in testosterone secretion and the expression of genes involved in steroidogenesis, such as StAR and CYP11A1, following peptide treatment.
Laboratory notes
- When using with Leydig cell lines like MA-10, hCG or cAMP analogs are often used as positive controls for stimulating steroidogenesis.
- Testosterone output is typically measured from the culture medium using specific ELISA or LC-MS/MS methods.
- Dissolve the lyophilized peptide in sterile, nuclease-free water or an appropriate cell culture medium.
Storage and reconstitution basics: storage guide.
Questions about Testoluten
What is the structure of Testoluten?
Testoluten is a tripeptide with the amino acid sequence L-Lysyl-L-Glutamyl-L-Aspartic acid (Lys-Glu-Asp).
Which cell types are used to study its activity?
Primary Leydig cells or immortalized murine Leydig cell lines like MA-10 and TM3 are common in-vitro models for studying its effects.
What is a key gene marker to assess its effect on steroidogenesis?
The expression of the Steroidogenic Acute Regulatory Protein (StAR) gene is a critical, rate-limiting step in steroid hormone production and is often measured via qPCR.
Is Testoluten for human use?
No. It is supplied strictly for in-vitro laboratory research and is not for human or animal consumption.
Related research peptides
All products are sold for laboratory research use only. Not for human consumption, not a medicine, not for diagnosing, treating or preventing any condition.