Antimicrobial · Antimicrobial Peptide (AMP)
Aurein 1.2
Aurein 1.2 is a 13-amino acid antimicrobial peptide from Australian tree frogs, used in research to study bacterial membrane permeabilization.
- Length
- 13 amino acids
- Half-life
- Not well characterised
- Mechanism
- Aurein 1.2 is an amphipathic alpha-helical peptide that disrupts microbial cell membranes through pore formation or the carpet mechanism.
- Studied in
- In microbiology labs, it is used as a model peptide to investigate mechanisms of bacterial membrane disruption and to assess antimicrobial efficacy.
About Aurein 1.2
Aurein 1.2 is a cationic antimicrobial peptide (AMP) originally isolated from the skin secretions of the Australian green and golden bell frog, Litoria aurea. This short, 13-residue peptide (GLFDIIKKIAESF-NH2) adopts an amphipathic α-helical conformation upon interaction with lipid membranes. Its simple structure and broad-spectrum activity make it a frequently studied example of a host defense peptide.
The peptide's mechanism of action involves the electrostatic attraction to negatively charged microbial membranes, followed by membrane insertion and disruption. This can occur via several proposed models, leading to membrane depolarization and cell death. It is used in biophysical and microbiological studies to examine peptide-lipid interactions and to determine minimum inhibitory concentrations (MICs) against panels of bacteria.
Laboratory notes
- Activity of Aurein 1.2 can be sensitive to salt concentration; ensure experimental buffers are consistent with those reported in relevant literature.
- To assess membrane permeabilization, researchers often use fluorescent dye-leakage assays with liposomes or live/dead staining assays with bacterial cultures.
- Peptide solutions should be prepared in low-adhesion microcentrifuge tubes, as AMPs can adsorb to standard plastic surfaces.
Storage and reconstitution basics: storage guide.
Questions about Aurein 1.2
What is the origin of Aurein 1.2?
Aurein 1.2 was first isolated from the skin secretions of the Australian tree frog, Litoria aurea. The research compound is a synthetic version.
How does Aurein 1.2 exert its antimicrobial effect?
It disrupts the integrity of bacterial cell membranes due to its amphipathic α-helical structure, leading to cell lysis.
Why is Aurein 1.2 sold for research use only?
Regena products, including Aurein 1.2, are intended exclusively for in-vitro laboratory research and are not for human or animal consumption or administration.
Is Aurein 1.2 for human use?
No. It is supplied strictly for in-vitro laboratory research and is not for human or animal consumption.
Related research peptides
All products are sold for laboratory research use only. Not for human consumption, not a medicine, not for diagnosing, treating or preventing any condition.