Other · Synthetic analogue of a mitochondrial-derived peptide
S14G-humanin (HNG)
Also known as HNG; [Gly14]-humanin
A Ser14-to-Gly humanin analogue used to investigate mitochondrial stress signalling and apoptosis-related pathways.
- Length
- 24 amino acids
- Half-life
- Not well characterised
- Mechanism
- Humanin-family signalling involves a proposed CNTFRα–WSX-1–gp130 receptor complex and formyl peptide receptor 2 (FPR2), with downstream responses that depend on the experimental system. HNG is a Ser14-to-Gly analogue used to probe these pathways; intracellular humanin interactions with apoptosis-related proteins such as BAX provide an additional mechanistic context.
- Studied in
- HNG is studied in neuronal cell cultures, isolated mitochondria and rodent models examining cellular stress, apoptosis and mitochondrial function.
About S14G-humanin (HNG)
S14G-humanin is a synthetic analogue of humanin, a peptide originally identified in 2001 through a functional screen of a cDNA library derived from brain tissue. Its defining modification replaces serine at position 14 with glycine in the conventional 24-amino-acid humanin sequence. This single substitution produced greater activity than the parent peptide in several early cellular assays, making HNG a widely used experimental comparator. The modification does not add a lipid group or extend the peptide chain.
Humanin biology spans extracellular receptor signalling and intracellular interactions with apoptosis-associated proteins. For HNG experiments, the proposed CNTFRα–WSX-1–gp130 complex, FPR2 and downstream STAT3 signalling are relevant mechanistic candidates, but their involvement should be established in the chosen model. Neuronal stress assays and mitochondrial readouts feature prominently in the literature. Differences in receptor expression, peptide preparation and stress stimulus can alter experimental responses, so activity reported for humanin should not automatically be assigned to every analogue.
Laboratory notes
- Confirm the Ser14-to-Gly substitution and terminal chemistry against the analytical certificate; parent humanin is not an interchangeable identity control.
- The sequence contains cysteine and methionine; monitor oxidation and possible disulphide-linked species when assessing preparation stability.
- For research use only. Include parent humanin, a suitable sequence control and receptor-pathway controls when interpreting cellular responses.
Storage and reconstitution basics: storage guide.
Questions about S14G-humanin (HNG)
What distinguishes HNG from unmodified humanin?
HNG replaces the serine at position 14 with glycine while retaining the conventional 24-residue length.
Is HNG selective for one receptor?
It should not be treated as a validated single-receptor-selective probe. Humanin-family studies implicate multiple receptor systems and intracellular binding partners.
Why can HNG results differ between cell lines?
Receptor abundance, intracellular signalling competence, stress conditions and peptide integrity can all affect the measured response.
Is S14G-humanin (HNG) for human use?
No. It is supplied strictly for in-vitro laboratory research and is not for human or animal consumption.
Related research peptides
All products are sold for laboratory research use only. Not for human consumption, not a medicine, not for diagnosing, treating or preventing any condition.