Regenerative · Recombinant enzyme
Sh-Polypeptide-50
Also known as Recombinant Human Superoxide Dismutase 1 (SOD1)
A recombinant form of human superoxide dismutase 1 (SOD1), an enzyme used in lab research to study and control oxidative stress pathways.
- Length
- 154 amino acids
- Half-life
- Not well characterised in vitro; context-dependent.
- Mechanism
- Catalyzes the dismutation of superoxide radicals into molecular oxygen and hydrogen peroxide.
- Studied in
- Used in cell culture systems to study the mitigation of oxidative stress and its downstream cellular consequences.
About Sh-Polypeptide-50
Sh-Polypeptide-50 is the INCI designation for a synthetically produced recombinant polypeptide identical to human Copper/Zinc Superoxide Dismutase (SOD1). SOD1 is a primary antioxidant metalloenzyme that exists as a 32-kDa homodimer, with each subunit containing one copper and one zinc atom. These metal ions are essential for its catalytic activity.
The enzyme's function is to catalyze the dismutation of the superoxide anion (O2•−), a reactive oxygen species, into ordinary molecular oxygen (O2) and hydrogen peroxide (H2O2). In laboratory research, it is added to cell culture media or other biological systems as a tool to quench superoxide radicals. This allows investigators to study the specific role of this radical in oxidative stress-induced signaling and cell damage.
Laboratory notes
- The catalytic activity of the enzyme requires the presence of copper and zinc ions; ensure appropriate buffer composition.
- Enzyme activity can be quantified using standard assays such as the nitroblue tetrazolium (NBT) reduction inhibition assay.
- Avoid exposing the reconstituted enzyme to strong denaturants or extreme pH, which can cause irreversible loss of activity.
Storage and reconstitution basics: storage guide.
Questions about Sh-Polypeptide-50
What does 'sh-Polypeptide' mean?
The 'sh-' prefix is an INCI designation that stands for 'synthetic human,' indicating it is a recombinant polypeptide with a sequence identical to a human protein.
What is the enzymatic reaction catalyzed by SOD1?
SOD1 catalyzes the reaction: 2O2•− + 2H+ → H2O2 + O2. The resulting hydrogen peroxide is then typically detoxified by catalase or glutathione peroxidase.
How is this polypeptide used in a laboratory setting?
It is primarily used as an exogenous antioxidant enzyme in cell culture experiments to scavenge superoxide and dissect its role in various biological phenomena.
Is Sh-Polypeptide-50 for human use?
No. It is supplied strictly for in-vitro laboratory research and is not for human or animal consumption.
Related research peptides
All products are sold for laboratory research use only. Not for human consumption, not a medicine, not for diagnosing, treating or preventing any condition.