Cosmetic · Hexapeptide
Hexapeptide-9
Also known as Gly-Pro-Gln-Gly-Pro-Gln
A hexapeptide fragment of collagen used in laboratory models to study extracellular matrix synthesis and dermal-epidermal junction formation.
- Length
- 6 amino acids
- Half-life
- Not well characterised
- Mechanism
- This peptide is a fragment derived from collagen type I that stimulates the synthesis of dermal proteins, including various collagen types, in fibroblast cell cultures.
- Studied in
- Laboratory studies investigate its influence on extracellular matrix production, dermal-epidermal junction integrity, and overall tissue structure in 3D skin models.
About Hexapeptide-9
Hexapeptide-9 is a synthetic peptide with the sequence Gly-Pro-Gln-Gly-Pro-Gln. This sequence represents a fragment of human collagen, a protein critical for the structural integrity of the basement membrane. Its structure is intended to mimic natural collagen breakdown products that can signal cellular responses.
In vitro research employs Hexapeptide-9 to explore cellular signaling pathways related to tissue repair and synthesis. Experiments using human dermal fibroblasts demonstrate its ability to upregulate the expression and production of key structural proteins, such as collagen I, collagen IV, laminin-5, and integrins, which helps elucidate the mechanisms governing the maintenance of the dermal-epidermal junction.
Laboratory notes
- The peptide sequence contains proline, which can introduce conformational rigidity, a factor to consider in structural or binding studies.
- For gene expression analysis (e.g., qPCR), fibroblast cultures should be synchronized before treatment to reduce variability in results.
- When assessing protein synthesis (e.g., via Western Blot or ELISA), include appropriate positive and negative controls for collagen production.
Storage and reconstitution basics: storage guide.
Questions about Hexapeptide-9
What is the origin of the Hexapeptide-9 sequence?
Its sequence, Gly-Pro-Gln-Gly-Pro-Gln, is a fragment derived from the alpha chain of human collagen type I, a key structural protein in the extracellular matrix.
What cellular processes are studied using Hexapeptide-9?
Researchers use it to study the synthesis of extracellular matrix components, specifically different types of collagen and other structural proteins, in dermal fibroblast cell cultures.
How is the activity of Hexapeptide-9 typically measured in a lab setting?
Activity is often quantified by measuring changes in mRNA expression levels of collagen genes (e.g., COL1A1, COL4A1) via RT-qPCR or by measuring protein levels via ELISA or Western Blotting.
Is Hexapeptide-9 for human use?
No. It is supplied strictly for in-vitro laboratory research and is not for human or animal consumption.
Related research peptides
All products are sold for laboratory research use only. Not for human consumption, not a medicine, not for diagnosing, treating or preventing any condition.